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KMID : 0613820230330010050
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Journal of Life Science
2023 Volume.33 No. 1 p.50 ~ p.55
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Inhibitory Effect of Rosa davurica Pall. on LPS-mediated Nitric Oxide Production via NF-κB signaling
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Kwon Soon-Pyo
Lee Sun-Ryung
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Abstract
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This study was designed to determine the activities of Rosa davurica Pall. leaf extract and their regulatory mechanisms in macrophage inflammation. Anti-inflammatory potential of Rosa davurica Pall. leaf extract was evaluated by measuring the nitric oxide (NO) release and inducible nitric oxide synthase (iNOS) synthesis in lipopolysaccharide (LPS)-treated macrophage Raw 264.7 cells. Rosa davurica Pall. leaf extract potently inhibited LPS-induced NO release in a dose dependent manner. However, cell viability decreased to about 50% at high dose of 500 μg/ml, resulting in cytotoxicity. LPS-induced iNOS protein expression was also reduced significantly after treatment with Rosa davurica Pall. leaf
extract. Furthermore, extract of Rosa davurica Pall. attenuated LPS-mediated phosphorylation of IκB and nuclear factor (NF-κB). Suppression of NF-κB signaling by treatment with PDTC, an NF-κB specific inhibitor, accelerated the inhibition of NO production and iNOS protein expression. These results suggest that Rosa davurica Pall. exhibits the anti-inflammatory potential in LPS-induced macro- phage inflammation, partly through inhibition of NO production by down-regulation of NF-κB signaling.
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KEYWORD
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Inflammation, iNOS, NO, NF-κB, Rosa davurica Pall.
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